Not known Details About attune flow cytometer
Not known Details About attune flow cytometer
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At the moment, the early indications were dismissed as debris, however the retrospective evaluation shown shared qualities Using the problematic cells in culture. Tracing the an infection served the lab set up additional laboratory processes for screening and protection of assay-crucial cell lines.
No matter whether you’re a novice or an expert, investigate our resources to advance your analysis and expertise in flow cytometry.
(B) RAW 264.seven macrophages are sometimes annotated inaccurately with regard to particle rely due to their complexity and noncircular borders. The amount of singlets accessible for Investigation boosts as soon as the person trains and employs a more exact design.
The award-winning Invitrogen Attune CytPix Flow Cytometer brings together acoustic focusing with a higher-velocity brightfield digital camera for simultaneous superior throughput flow cytometry and significant resolution brightfield imaging.
"A picture just isn't pretty really worth one thousand dots, but it really really helps to see photographs. It is excellent to view images that correlate with staining. For example, AnnexinV-good cells which might be morphologically various than healthy cells, or CD14+ monocytes which are larger sized and a lot more textured than CD3+/CD4+/CD14- lymphocytes.
In this article, a highly skilled user has gated singlets confidently. Just after assessing the guide singlet gate, the CytPix picture-derived parameter ParticleCount reveals this gate is made up of over four% aggregates.
Pair your Attune flow cytometer with the autosampler for increased workflow effectiveness, and insert robotic integration for maximum throughput.
Our researchers will design and style panels for yourself as many times you would like—totally free, no product or service acquire wanted.
Early indications of this contamination experienced to begin with been dismissed as particles. Backgating to find out new populations. Backgating imaged cells about the Attune CytPix Flow Cytometer also lets you use morphological capabilities to find intriguing subpopulations that would not be evident from flow cytometry information on your own.
Probably most of all, these activities include cells of Plainly unique phenotypes which could lead to incorrect conclusions concerning double positive activities (particularly in scarce populations).
Seem waves help precise cell alignment, resulting in Remarkable facts top quality and ~10X quicker sample flow premiums.
We Formerly shown the power of imaging Auto-T/Ramos mobile interactions. Permit’s check out just the population of greatest curiosity, the double beneficial situations, To find out more. We are able to now use prolonged morphological parameters (listed here, circularity and skewness of intensity) to further more examine the options of those populations and refine gating on these events, increasing knowledge more info robustness.
Leverage the power of Highly developed technological innovation and innovation to accelerate your discoveries. Trust inside the Attune Flow Cytometer spouse and children to deliver the reliability, velocity, and precision that established your perform apart.
Adaptable payment plan from Agilent Economical Alternatives permit you to get the essential instrumentation you'll need for fast venture, or to develop your lab.
At the guts of each and every Attune Flow Cytometer lies our groundbreaking acoustic focusing technologies, which utilizes audio waves to meticulously align cells in the flow cell. This revolutionary technique surpasses standard hydrodynamic methods, causing a lot quicker processing speeds, Outstanding facts good quality, and increased sample throughput.
The Attune Xenith cytometer can be a higher-parameter spectral analyzer, supplying equally spectral unmixing and standard flow capabilities for comprehensive and multipurpose mobile Investigation.
Agilent Benefit Guarantee guarantees you no less than a decade of instrument use through the date you purchase. Otherwise, we will credit you With all the residual worth of the technique towards an upgraded product.
What gave the impression to be a homogeneous population is actually extra assorted—an insight that should be viewed as when interpreting results.